The synthesis of radioactive cholesterol and fatty acids in vitro.
نویسندگان
چکیده
It has been established that rat liver slices are able to convert acetate (l), acetone (2), pyruvate (3, 4), butyrate, hexanoate, and octanoate (4) to cholesterol and long chain fatty acids. Zabin and Bloch (5) and Price and Rittenberg (6) administered 1 ,3-C14-acetone to rats and demonstrated that it is converted to fatty acids and cholesterol. The former investigators (5) also reported that methyl-labeled isovalerate is a good source of carbon for the biosynthesis of cholesterol .by the intact animal. In order to determine the ability of surviving liver tissue to utilize various substrates for these biosynthetic processes, and with the hope that some insight might thus be gained into the mechanisms involved, we have investigated the behavior of a number of labeled precursors. The following substances were incubated with liver slices and the relative degree of incorporation of isotope into cholesterol and fatty acids was determined: carboxyl as well as methyl C14-acetate, carbonyl C14-acetone, methyl C14isovalerate, doubly Iabeled acetaldehyde and acetate, and preparations of acetoacetate labeled in the carboxyl-, carbonyl-, and methyl-methylene positions, respectively. In every experiment, the substance under investigation was compared with labeled acetate simultaneously incubated with slices obtained from the same liver tissue.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 189 1 شماره
صفحات -
تاریخ انتشار 1951